|
KMID : 0356620150300020216
|
|
Journal of Korean Society of Endocrinology
2015 Volume.30 No. 2 p.216 ~ p.220
|
|
|
Glucagon-Like Peptide-1 Increases Mitochondrial Biogenesis and Function in INS-1 Rat Insulinoma Cells
|
|
:Kang Mi-Yeon
:Oh Tae-Jung/:Cho Young-Min
|
|
|
Abstract
|
|
|
|
Glucagon-like peptide-1 (GLP-1) is a gut-derived incretin hormone that increases glucose-stimulated insulin secretion in pancreatic β-cells. Since mitochondrial function is crucial to insulin secretion, we hypothesized that GLP-1 may increase mitochondrial biogenesis in pancreatic β-cells. We treated INS-1 rat insulinoma cells with GLP-1 or exendin-4 for 48 hours and measured mitochondrial mass and function. Both GLP-1 and exendin-4 increased mitochondrial mass by approximately 20%. The mitochondria/cytosol ratio was increased from 7.60±3.12% to 10.53±2.70% by exendin-4. In addition, GLP-1 increased the mitochondrial membrane potential and oxygen consumption. Proliferator-activated receptor-gamma coactivator 1α expression was increased approximately 2-fold by GLP-1 treatment. In conclusion, the present study presents evidence for a new mechanism of action by which GLP-1 improves pancreatic β-cell function via enhanced mitochondrial mass and performance.
|
|
|
KEYWORD
|
|
|
Glucagon-like peptide-1, Mitochondria, Diabetes mellitus, Beta cell, Insulin
|
|
|
FullTexts / Linksout information
|
|
|
|
|
Listed journal information
|
|
  
|